Sample Requirements for Evidence Submission 2017-09-12T18:08:34+00:00

Sample Requirements for Evidence Submission

Forensic DNA

1. Sexual Assault

a. Living victim:

  • High vaginal swab taken within 72 hours of sexual activity (mandatory primary evidence)
  • Reference blood sample of victim (mandatory)
  • Reference blood sample of accused persons (mandatory)
  • Anal swab taken within 24 hours of sexual activity, wherever applicable
  • Clothing of the victim (optional supportive evidence)
  • Condom (if recovered from place of occurrence)

b. Deceased Victim:

  • High vaginal swab taken within 24 hours of death provided sexual activity occurred moments before death (mandatory primary evidence)
  • Reference bone/teeth/nail sample of victim (mandatory)
  • Reference blood sample of accused persons (mandatory)

c. Dead Body Identification/Mass Disaster Victim Identification

  • Clean and dry Bone OR plucked nails OR plucked teeth or plucked hair (in the same order of preference)
  • Reference blood samples of claimant parents or offspring

c.Parentage Analysis

  • Blood sample (or buccal swab)of disputed child OR reference bone sample of child, if deceased
  • Reference blood samples of parents

d. DNA cross matching

  • Original exhibit containing blood, semen or saliva stain if the exhibit is portable OR swab taken from the place of occurrence
  • Reference blood samples of subjects involved

e. DNA profiling

  • Clean and dry Bone OR plucked nails OR plucked teeth or plucked hair (order of preference)

Note: The service of DNA profiling is currently available only for suicider’s samples.


  1. Comparisons:
  • Cartridge with Cartridge: All the Cartridges to be compared are required
  • Cartridge with weapon: All the cartridges along with unloaded weapon + 5 live rounds if weapon is handgun and 10 live rounds if weapon is long barrel for test purpose
  • Bullet with weapon: Original crime bullet recovered along with weapon + 10 live rounds for test purpose
  • bullet with bullet: All the original bullets to be compared are required
  1. Gunshot residue analysis (for distance determination):
  • Original exhibits suspected to have gunshot residue
  • Weapon of offence
  • 10 live rounds for test purpose
  1. Serial number restoration on weapon
  • Original weapon of offence
  1. Workability of weapon
  • Original weapon of offence
  • 5 live rounds for test purpose
  1. Tool marks examination
  • Original tool or active part of the tool e.g. blade, edge, sawtooth etc.
  • Original Exhibit having tool marks, if portable OR silicon mould of the tool mark, if original exhibit is not portable
  • Scaled photographs of the tool mark (taken before transportation)


  1. Engine/chassis number
  • Vehicle to be analysed should be parked at NSFA premises till the completion of analysis
  • Auto mechanic if the engine/chassis number is not accessible for inspection/analysis


  1. Pre-blast material analysis:
  • 1-2 teaspoon specimen sample of unknown chemical/explosive
  • Small piece of detonating cord
  • Different parts of IED’s (battery, electronic circuit, wires, ball bearings etc.)
  1. Post blast material analysis:
  • Swabs taken from seat of blast and probable areas (all sealed separately)
  • Ball bearings and different fragments of IED
  1. Triggering mechanism of IED’s
  • Container
  • Wires
  • Batteries
  • Electronic circuit
  • Mechanical connectors


  1. Development of latent prints
  • Original exhibit containing any latent prints
  1. Manual comparisons
  • Questioned fingerprints on exhibit (original)
  • Reference rolled fingerprints of accused person(s) on ten-print card taken by competent authority using appropriate ink


  1. Handwriting/signature analysis
  • Questioned document in original
  • Specimen handwriting/signature (duly taken) in slow, medium and fast pace, 3 pages each in prescribed method, by competent authority
  • Maximum number of admitted routine handwriting/signatures (all original) of the accused written by him before the date of writing the questioned handwriting/signature
  1. Ink comparison/alteration/erasures/obliteration:
  • Original questioned item
  • Original normal course of writing (diaries, exam papers, previous registers etc.) in case of erasure
  1. Indented writing
  • Original Document suspected to have indentations
  1. Charred documents analysis
  • Original charred document
  1. Security features of documents
  • Original document whose veracity is under question
  • Reference authentic specimen document bearing security features

Seals of the evidence/samples:

Seals of evidence/samples and seal patterns are checked. If the seal pattern is not in accordance with those mentioned in covering letter or not intact/broken, case is returned. If seals are partially intact, samples are accepted mentioning the condition of the seals.

Quality and Quantity of the sample:

The quality and quality of the evidence/samples is checked by the concerned lab in presence of ERU official. The final decision of acceptance, partial acceptance or rejection of the case on the basis of evidence rests upon the officer Incharge of concerned lab.


Case Submission form

It covers information about the sender, FIR No., PPC clause imposed, Police station, contact numbers etc. Signature are taken from the special messenger/evidence carrier  with name, designation and belt number on this document that the evidence is being handed over to NFSA.


The receiving of the case is given on “Road Certificate” is given.


If the case is partially acceptable (having some reservation) on screening, an objection letter is sent to case submitting authority informing about necessary documents/additional samples which are essential for further processing of the case. A receipt is given to the person bringing the samples along with the objection letter and receiving on Road Certificate.


On initial screening, if the samples are not acceptable, ERU rejects the case and sends a letter of information to the referring authority about the reasons of rejection. Description of evidence and messenger name is also mentioned on the back side of road certificate.


If a case is received through courier, scrutiny of documents and sample is done on the same lines as mentioned above.

If the case is not acceptable, a letter is forwarded to the concerning authority showing all the reasons of case rejection and all the evidence is couriered back.


In case of additional samples/ remaining samples of a case received, a new summary form is filled in which new samples are noted and a receiving on road certificate is given to the submitting person.